Fig. 7. MOM recruitment assay with MOM-N-cadherin. Cells were co-transfected with MOM-N-cadherin and each mARVCF isoform as an EGFP-fusion plasmid. As an example, EGFP-FL-3/7 is shown in MCF7 cells, demonstrating the inhomogeneous interaction pattern between all of the mARVCF isoforms and the cytoplasmic domain of N-cadherin obtained in every cell line. (A) Indirect immunofluorescence with monoclonal birch profilin (BP) antibodies visualising the MOM-N-cadherin construct. (B) Localisation of EGFP-FL-3/7. (C) Merged image of (A) and (B). (D) 1.5x103 MCF7 cells expressing MOM-N-cadherin and EGFP-FL-3/7 were counted. In 36.7% of these cells a mitochondrial located interaction of both constructs was detected. In 63.3% of cells expressing MOM-N-cadherin and EGFP-FL-3/7 such an interaction did not take place. Error bars indicate standard deviation. Bar, 10 µm.
