Fig. 1. Dynamics of GFP-ensconsin chimera speckles in vivo. Fluorescent speckle microscopy (FSM) was used to record the dynamics of 5xGFP-EMTB chimeras in living cells and cytoskeletons. (a-e) Time-lapse micrographs of 5xGFP-EMTB speckles in living cells. Arrows point to speckles that remain from one interval to the next, while arrowheads denote speckles that appear or disappear during the sequence. The bar in the right-hand corner of time-lapse series e shows the magnification of 1µm in all panels. (f-j) Kymographs of a single MT segment from the time-lapse sequences. As shown, a and f are from untreated, b and g, are from detergent-extracted, c and h are from ATP-reduced, d and i are from ATP-reduced and then recovered and e and j are from Taxol-treated cells. See Materials and Methods for details of the treatments.
