Fig. 3. Photobleaching and recovery of ensconsin chimera in detergent-extracted cells. (A) Time-lapse micrographs of the bleached area are shown before (prebleach), at the time of bleaching (white circle, showing the position of the laser bleach, since no image was captured at the instant of the bleach) and at intervals during the recovery. Elapsed time, in seconds, is shown in each micrograph. (B) Corrected fluorescence of a bleached MT (pixel intensity) was plotted against time (in seconds) at intervals before and after bleaching. The time of the bleach is marked with an arrow; fluorescence shown has been corrected, using an unbleached segment of an adjacent MT, for decay caused by photobleaching during repeated illumination, and cellular background has been subtracted.
