Fig. 5. Dynamics of MAP interaction with MTs does not depend upon their state of post-translational detyrosination. Micrographs of fixed TC-7 cells show fluorescence of MAP chimera visualized with GFP(a,c,e,g) and immunofluorescence of detyrosinated tubulin (anti-Glu antibody) visualized with rhodamine secondary antibody(b,d,f,h). Cells were treated as follows: a,b, untreated; c,d, detergent-extracted; e,f, ATP-reduced (azide-treated); and g,h, Taxol-treated. Note that, although there are more detyrosinated MTs in treated cells than in untreated ones, the detyrosinated MTs constitute only a subset of the total array under all conditions. Bar, 10 µm.
