Fig. 2. Microfilament organisation in HEK 293 cells transfected with acidic calponin. Panels A and B illustrate untransfected control cells and ac.CaP-GFP-transfected cells stained with Texas Red-X phalloidin, respectively. The organisation of microfilaments is dramatically altered in ac.CaP-GFP cells (C). Indeed, microfilaments are thicker and display a twisted organisation compared with control cells. Panel D corresponds to the overlay of B and C. The yellow color indicates that ac.CaP-GFP mainly binds to F-actin. Note the accumulation of calponin and microfilaments within the tip of the process (see arrows). Bar, 15 µm.
