Fig. 3. Microtubule organisation in HEK 293 cells transfected with acidic calponin. Panels A and B illustrate the same control cells double stained with Texas Red-X phalloidin and ß-tubulin antibodies, respectively. Panel C corresponds to the overlay of A and B. It shows that microtubules radiated through the cytoplasm as individual filaments until stopped by the cortical actin filaments. Panel D shows an ac.CaP-RFP-transfected cell. Panel E illustrates the same cell counterstained with ß-tubulin antibodies. Panel F corresponds to the overlay of D and E. It shows that ac.CaP-RFP-transfected cells displayed a clear-cut reorganisation of microtubules compared with control cells. Thick bundles of microtubules were observed within the cell bodies and extensions. Moreover, the ac.CaP-RFP (in red) was not co-distributed with microtubules (in green) but rather adjacent. Panel G shows a process of an ac.CaP-RFP cell. Panel H illustrate the same process counterstained with ß-tubulin antibodies. Panel I corresponds to the overlay of G and H. It shows that ac.CaP filaments/microfilaments (in red) and microtubules (in green) clearly form ‘curves’ within this process (see arrows). Bar, 15 µm.
