Fig. 3. The expression of mdrA2 is developmentally regulated. A northern blot of total cellular RNA isolated from DH1 cells at 2.5 hour intervals after starvation on filter pads was probed with a radiolabelled fragment of the mdrA2 gene (A). A western blot of lysates from 1x10⁵ cells of mdrA1^-/mdrA2^-, DH1, rtoA^- and rtoA^-/mdrA1^-/mdrA2^- was probed with affinity-purified anti-MdrA2 peptide antibodies (B). A similar western was performed using 1x10⁵ cells of DH1 at 2.5 hour intervals after starvation on filter pads (C). mdrA^–, mdrA1^-/mdrA2^-; rtoA^–/mdrA^–, rtoA^-/mdrA1^-/mdrA2^-.

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