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Fig. 2. Wild-type GHR cells incubated for 30 minutes with GH were double immunolabeled for GH (15 nm gold) and clathrin (5 nm gold) to unequivocally identify coated areas. (A) GH was found at non-coated areas of the plasma membrane as well as in flat clathrin-coated lattices (arrowheads). (B) In addition, GH was regularly seen in clathrin-coated pits (arrow). P, plasma membrane. Scale bars: 200 nm.





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