Fig. 4. Cryosections of GHR-F327A cells incubated for 30 minutes with GH, showing the exclusion of GH from clathrin-coated pits and vesicles. (A) GH (15 nm gold) accumulates at the plasma membrane (P). (B) GH (15 nm gold) can also be found associated with clathrin-coated lattices (arrowheads) at the plasma membrane, which positively label for clathrin (5 nm gold). The arrows point to clathrin-coated vesicles. (C) GH (15 nm gold) is found in a clathrin (5 nm gold) -coated lattice (arrowhead), but not in the clathrin-coated pit (arrow). (D) GH (10 nm gold) and (E) GH (15 nm gold) are two more examples, showing that GH is excluded from clathrin-coated pits (arrow). Scale bars: 200 nm.
