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COS-7 cells were transfected with expression vectors encoding GFP-FENS-1 and processed for live image recording of GFP-fluorescence as described in Materials and Methods. 100 nM wortmannin was added at the times indicated.

COS-7 cells were transfected with expression vectors encoding GFP-i FYVE FENS-1 and processed for live image recording of GFP-fluorescence as described in Materials and Methods. 100 nM wortmannin was added at the times indicated.

COS-7 cells were transfected with expression vectors encoding GFP-DFCP1 and processed for live image recording of GFP-fluorescence as described in Materials and Methods.

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