Fig. 7. Colocalisation of exogenously expressed DFCP1 with ER and Golgi markers. Top panels: COS-7 cells were co-transfected with expression vectors encoding GFP-elastase and Myc-DFCP1. Cells were fixed with paraformaldehyde and processed for direct (GFP) or indirect (Texas Red, anti-Myc) fluorescence as described in the Materials and Methods. The images shown were taken at high magnification with a confocal section of <1 µm on a UltraView confocal microscope. Lower panels: COS-7 cells were transfected with an expression vector encoding GFP-DFCP1. Cells were fixed with paraformaldehyde and processed for direct (GFP) or indirect (Texas-Red, anti endogenous giantin) fluorescence as described in the Materials and Methods.
