Fig. 8. Effect of exogenous expression of Myc-DFCP1 and Myc-[C647S/C770S]DFCP1 on the morphology of the Golgi. COS-7 cells were transfected with expression vectors encoding Myc-DFCP1, Myc-[C647S/C770S]-DFCP1 (DFCP1(DM)) or the Myc-tagged tandem FYVE-domains of DFCP1 (iFYVE DFCP1; see inset). Where indicated, cells were treated with 2 µg ml-1 brefeldin A (BFA) for 20 minutes before fixation. Cells were fixed with paraformaldehyde and processed for indirect immunofluorescence (fluorescein, anti-Myc; Texas-Red, anti endogenous giantin) as described in the Materials and Methods. Cells expressing Myc-DFCP1 or Myc-DFCP1(DM) are marked with an asterisk in the red, giantin images. The arrows mark a cell expressing relatively low levels of Myc-DFCP1 and consequently show good colocalisation of Myc-DFCP1 and an intact, giantin-containing compartment.
