Fig. 3. Immunoblot analysis of C2C12 cell lysates. Cell lysates were prepared from C2C12 cells kept in differentiation medium for 0-3 days and samples (50 µg protein) were resolved by 10% SDS-PAGE, transferred to nitrocellulose filters and immunoblotted with antibodies to lamins and myosin. A representative gel stained with Coomassie Blue is also shown (CB). Molecular mass markers indicated on the left (from top to bottom) are: phosphorylase b, 94 kDa; albumin 67 kDa; ovalbumin, 43 kDa; and carbonic anhydrase, 30 kDa.
