Fig. 4. Localization studies in extracted cells. C2C12 myoblasts (MB) and myotubes (MT) were extracted with detergents, digested with nucleases and treated with 2M NaCl, followed by formaldehyde fixation and labeling with (A) mAb LA-2H10, (B) mAb LA-2B3, (C) mAb SC-35 and (D) m3G antibody to capped RNA, and with DAPI. Unextracted cells are shown as controls. Images of optical sections of 1 µm are displayed. Scale bar: 10 µm.

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