Fig. 7. Cx32 does not colocalize with Cx43/ß-gal. Control (con; A-C), 43ß1 (D-F) and 43ß2 (G-I) cells were transiently transfected with Cx32 and allowed to recover for 48 hours. The cells were fixed, permeabilized and then double-label immunostained using mouse anti-ß-gal (A,D,G) and rabbit anti-Cx32 (B,E,H) antisera, which were detected using Texas Red-conjugated goat anti-mouse IgG and FITC-conjugated goat anti-rabbit IgG as secondary antibodies, respectively. The intracellular distribution of Cx32 was not affected by Cx43/ß-gal expression (arrows). Bar, 10 µm.
