Fig. 1. GFP-Rab22a localizes to early and late endosomes. CHO cells were transfected with pEGFP-Rab22aWT and 24 hours later the distribution of the GFP fluorescence was recorded. Early endosomes were labeled by a 5 minute uptake of Rh-HRP (A-C) or Rh-Tf (D-F). Late endosomes were labeled by a 5 minute uptake of Rh-HRP followed by 15 minute chase (G-I) or by immunostaining with an anti CI-M6PR antibody (J-L). Notice the large size of some Rab22a-positive vacuoles (arrow heads) and the altered morphology of early and late compartments in cells overexpressing Rab22a (t) when compared with untransfected cells (u). The inset in B shows tubular projections attached to a large vesicle. The bars represent 7 µm.
