Fig. 1. Expression of a human HIF-1 mRNA isoform induced by the zinc ion. HEK 293 cells were cultured for 4 hours in the absence (C) and presence of ZnCl₂ (100, 300 and 500 µM) (Zn), CoCl2 (100 µM) (Co), or NiCl2 (300 µM) (Ni), or subjected to 4 hours of hypoxia (H). HIF-1, its isoform, and ß-actin mRNAs were amplified using RT-PCR, and analyzed by electrophoresis and ethidium bromide staining, as described in Materials and Methods (A, B, C). The molecular size of PCR product was assessed by comparing its mobility with those of DNA markers (M). These mRNA levels were analyzed by semiquantitative RT-PCR using [-³²P]CTP (D). The relative amounts of the mRNAs were expressed as the ratios of the cpm value of HIF-1 or its variant fragments to ß-actin under each set of conditions (E). The results presented in each panel represent three separate experiments.

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