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Fig. 10. Co-immunoprecipitation of HIF-1{alpha}Z and ARNT. pARNT plasmid was transfected into stable cell lines transfected with pcDNA and pHA-HIF-1{alpha}Z plasmids. After 4 hours of hypoxia, the cytosolic proteins were immunoprecipitated (IP) by rabbit anti-HA antibody ({alpha}-HA) or goat anti-ARNT antibody ({alpha}-ARNT). HA-HIF-1{alpha}Z protein and ARNT protein were identified by western blotting, and are indicated by arrows.





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