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Fig. 4. Subcellular localization of HIF-1{alpha} and HIF-1{alpha}Z. HEK 293 cells (left panel) and pHA-HIF-1{alpha}Z transfectant cells (right panel) were subjected to normoxia or 4 hours of hypoxia, and then incubated with rabbit anti-HIF-1{alpha} antibody and rabbit anti-HA antibody, respectively, followed by a FITC-conjugated goat anti-rabbit antibody. The corresponding transmission images are shown in the outer columns.





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