Fig. 7. Quantification of the effect of MPB-07 on location of wild-type and delF508-CFTR in epithelial cells from nasal brushings. Cells were examined (up to 140 cells from each brushing) and categorised as having a distinct CFTR location of either predominantly apical (filled bars) or perinuclear (open bars). A and B show wild-type cells, C and D show delF508/delF508 cells; A and C are untreated, and B and D are MPB-07 treated. Cells showing intermediate distributions that were either a discrete location adjacent to the nucleus, characteristic of delF508 cells (juxtanuclear), or more evenly distributed throughout the cell including the apical region were counted but were not included in the histogram for clarity (see text). The data are mean±s.e.m. for cells from five non-cystic fibrosis and four delF508/delF508 cystic fibrosis individuals (>100 cells counted). *P<0.001; **P<0.0001 for difference from untreated delF508 cells as assessed by Student’s t-test.
