Fig. 4. Sub-cellular distribution of actinin-4, actinin-1 and BP180 in pp126 oral keratinocytes maintained in low calcium medium. Cells were processed for immunofluorescence microscopy and labeled with antibodies against actinin-4 (A), actinin-1 (B) and BP180 (C). Actinin-4 localizes to the cell-edge in membrane ruffles in A while actinin-1 is present in focal contact-like structures and along the cortical microfilament cytoskeleton in B. By contrast, BP180 localizes along the basal aspect of the cell (C). Bars, 10 µm.
