Fig. 8. BP180 co-distributes with p120 catenin in MCF-10A cells and pp126 oral keratinocytes. The localization of p120 catenin and BP180 proteins was assessed in MCF-10A cells and pp126 oral keratinocytes maintained in 2.0 mM calcium for 4 hours. Cells were processed for double-label indirect immunofluorescence microscopy using antibodies against p120 catenin (A,D) and BP180 (B,E). p120 catenin is present at cell-cell interaction sites in both cell types (short arrows in A,D) where it co-distributes with BP180 (short arrows in B,E). This is demonstrated by the yellow color in the overlays in C and F. Although BP180 antibodies also stain along the basal aspect of MCF-10A cells (long arrow in B) and in pp126 cells (long arrow in E), p120 catenin is absent from these sites (A,D). Bar, 10 µm.
