Fig. 4. (A-F) FISH with telomere, CEN and BAC probes applied to meiotic prophases of the asy1 meiotic mutant. (A-D) FISH with telomere (red) and CEN (green) probes to show unpaired telomeres in meiotic interphase (A), paired telomeres in leptotene (B,C) and unpaired (dissociated) telomeres in the asynaptic post-leptotene stage (D). B and C show dual and triple filter images of the same leptotene nucleus. (E,F) Dual FISH with telomere (red) and BAC T9J23 (green) probes to show variable pairing (E) or unpairing (F) of the BAC in post-leptotene asynaptic nuclei. Bar, 10 µm.
