Fig. 4. Expression patterns of GFP-N-RAP-LIM (A,C) and of 
-actinin in the same fields (B,D). Cardiomyocytes were transfected with GFP-N-RAP-LIM at 4 days and viewed 3 days post transfection (A,B) or transfected at 2 days and viewed 1 day post transfection (C,D). In cardiomyocytes transfected after 4 days in culture, GFP-N-RAP-LIM was targeted to the cell periphery and sites of cell to cell contact (arrowheads, A,B). Punctate or filamentous -actinin staining revealed that myofibril precursors were present in these cardiomyocytes, but did not contain GFP-N-RAP-LIM (arrows, A,B). In cardiomyocytes transfected after 1 or 2 days in culture, both GFP-N-RAP-LIM and -actinin were diffusely distributed (arrows, C,D), in contrast to neighboring untransfected cells exhibiting clear striations when stained for -actinin (asterisks, C,D).
