Fig. 6. The NCS, like the R-rings, contains calnexin, BiP and Nopp140. (A–C) Paraffin sections of human endometrium in the secretory phase, stained by double immunofluorescence for the ER transmembrane marker calnexin (A) and for Nopp140 (B and C, red panels). (A) and (B) were double labeled for the nucleolus with nucleolin and (C) for ER with BiP antibodies (green panels). The two images in each were merged. Some putative NCSs are indicated by arrows and two nucleoli are pointed out by arrowheads in (C). Bars, 10 µm. (D) Transmission electron micrograph of a deparaffinized section as in (A-C). The nucleolus (No), an adjacent NCS and the nuclear envelope (NE) are labeled. Note, the white holes in the nucleoplasm reflect the poor preservation of the paraffin sections. Bar, 0.5 µm.
