Fig. 1. Comparison of profilin amino acid sequences. (A) Multiple sequence alignment of the deduced amino acid sequence for CrPRF with plant, yeast, vertebrate and Vaccinia virus profilins. ClustalW analysis of the following sequences was performed using MacVector 7.0 software: Chlamydomonas reinhardtii (CrPRF; GenBank accession number AF335423), Arabidopsis thaliana profilin1 (AtPRF1; AAG10090), AtPRF2 (AAG10088), AtPRF3 (AAG10089), AtPRF4 (AAG10091), AtPFN4 (AAB39479), Zea mays profilin1 (ZmPRO1; X73279), ZmPRO2 (X73280), ZmPRO3 (X73281), ZmPRO4 (AF032370), ZmPRO5 (AF201459), Ricinus communis (RcPRO; AF092547), Schizosaccharomyces pombe (P39825), Saccharomyces cerevisiae (P07274), bovine profilin I (P02584), human profilin I (A28622) and Vaccinia virus profilin (P20844). Residues that are conserved in >51% of the displayed sequences are shown in bold and shaded grey. Gaps (-) were introduced to optimize the alignment. Conserved residues implicated in PLP binding are denoted by an asterisk, whereas those involved in actin binding are marked by a hash (#). The two regions of primary sequence that contribute to a plant-specific patch are overlined. Noteworthy substitutions that are predicted to affect CrPRF’s association with ligands are marked with a circle. (B) Phylogenetic comparison of the profilins shown in (A). The ClustalW multiple sequence alignment was analysed with a UPGMA algorithm and bootstrapped 1000 times using MacVector 7.0 software to create the tree shown here. Similar results were obtained using an neighbour-joining algorithm (not shown).
