Fig. 3. Dynamics of GFP-VASP during early events of phagocytosis. Raw264.7 macrophages expressing GFP-VASP were incubated with IgG-opsonised sheep RBC and followed by digital video microscopy. GFP-VASP begins to accumulate at the sites of engulfment approximately one minute after SRBC binding, remains associated with phagocytic cups during until the entire internalisation process and disappears upon closure of the phagocytic cups. The positions of bound sheep RBC were observed using DIC optics (not shown). Arrowheads indicate sites of sheep RBC binding that led to complete phagosome formation. Numbers represent elapsed time (in seconds) after sheep RBC settled onto cells. Scale bar: 10 µm.
