Fig. 4. Dynamics of GFP-Fyb/SLAP during early events of phagocytosis. Raw264.7 macrophages expressing GFP-Fyb/SLAP were incubated with IgG-opsonised SRBC and followed by video microscopy. GFP-Fyb/SLAP begins to accumulate at the sites of engulfment approximately one minute after sheep RBC binding, remains associated with phagocytic cups during the entire internalisation process and disappears upon closure of the phagocytic cups. The positions of bound sheep RBC were observed using DIC optics (not shown). Arrowheads indicate sites of sheep RBC binding that led to complete phagosome formation. Numbers represent elapsed time (in seconds) after SRBC settled onto cells. Scale bar: 10 µm.
