Fig. 5. Clostridium difficile toxin B inhibits the recruitment of VASP. Raw264.7 macrophages expressing GFP-VASP were incubated with 10 ng/ml C. difficile toxin B for two hours prior to use in phagocytosis assays with IgG-opsonised sheep RBC. Toxin B-treated cells bind sheep RBCs but do not internalise them (arrowheads in A). Note that there was no accumulation of GFP-VASP on the membrane abutting the bound particles (arrowheads in B). Scale bar: 10µm.

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