Fig. 8. Scanning electron microscopy analysis of the interaction between Raw264.7 cells and sheep erythrocytes. Untransfected Raw cells (A), cells expressing GFP-tagged ActA repeats (B), or the mutated GFP-tagged ActA repeats (C) were incubated with opsonised sheep RBC, fixed and processed for scanning electron microscopy. Raw cells expressing GFP-tagged ActA repeats were still able to bind to SRBC but did not form lamellipodia-like extentions, which, in contrast, are formed both by control cells and cells expressing the inactive form of the ActA repeats. Scale bar: 2 µm.
