Fig. 1. Centrosomal alignment (A,B) and distances between the centrosomes (C) in zygotes progressing into and through mitosis. (A) Fifteen populations were developmentally staged by assaying the percent of zygotes at or past metaphase; the alignment angles were measured on zygotes stained with anti-
-tubulin antibodies and the angle between the cell plate and the growth axis was measured on living embryos stained with FM® 4-64. Each symbol designates a different population and data points are the mean angles±s.e.m. in each population. (B) Distribution of alignment angles in one population of zygotes (A, grey circles). Each bar represents the mean from three timepoints±s.d. (C) Distances between centrosomes; bars indicate the mean of three populations±s.d.
