Fig. 6. Maximum intensity projections of deconvolved serial sections through control DFSF1 cells (A-D) and DFSF1 cells injected with expression vectors encoding GFPCoilin (E-H), GFPSMN (I-L) or YFPSmB (M-T). Control cells show weak, fine punctate staining with anti-p80-coilin (A) and anti-SMN (B) with no co-localization between the two (overlay in D). Endogenous Sm proteins show a speckled localisation (C). Over-expression of GFPCoilin leads to an increase in nucleoplasmic coilin (E, anti-p80 coilin; F, GFP-coilin; H, overlay), but not to formation of Cajal bodies. Endogenous snRNP proteins, detected with Y12, show a speckled nuclear localization (G). Overexpression of GFPSMN leads to fine punctate localization of GFPSMN, predominantly in the cytoplasm (J). No co-localization with p80-coilin (I) or endogenous Sm proteins (K) is seen. Over-expression of YFPSmB for 2 hours results in the formation of nuclear Cajal bodies (arrows in M-O) containing p80-coilin (M), SMN (N) and newly imported snRNP (O). In cells expressing YFPSmB for 16 hours, p80-coilin shows a fine punctate pattern (Q). SMN is seen in small nuclear bodies (arrows in R,T), but no longer shows co-localization with YFPSmB (S,T).
