Fig. 5. PEDF was the major tumor cell differentiation factor in human Schwann cell conditioned media. SK-N-BE(2) cells grown in (A) media alone, (B) media plus Schwann cell conditioned media; (C) media plus Schwann cell conditioned media and anti-PEDF antibody; (D) quantification of differentiation elicited by Schwann cell media, where 100% represents 55 out of 117 cells counted (open circles, media alone; closed circles, media plus anti-PEDF antibody); (E) purified rPEDF at the indicated concentrations were added to the growth media of SH-SY5Y neuroblastoma cells and differentiation quantified. 100% represents 150 differentiated cells/254 cells counted.
