Fig. 8. Interaction of lamin A mutants with the emerin N-terminal domain (EmC, residues 3-221). Lamin and EmC cDNAs were transcribed and translated in vitro and yielded ³⁵S-labeled translation products of the appropriate size (A). Single translation mixes containing identical levels of each lamin allele were subjected to immunoprecipitation with a guinea pig anti-lamin antibody coupled to Dynabeads. (B) Wild-type lamin A (WT) as well as each of the lamin mutants (LaA L85R, LaA N195K, LaA R482W and LaA L530P) were all efficiently immunoprecipitated with this antibody. Combined translation mixes (C) containing ³⁵S-labeled EmC and unlabeled lamin alleles were similarly immunoprecipitated. EmC was recovered in comparable amounts in wild-type lamin A and LaA R482W immunoprecipitates. Less efficient EmC recovery was observed with LaA L85R and LaA N195K. With LaA L530P, EmC was barely detectable. Unlabeled lamins were employed in (C) to avoid the EmC band being obscured by possible lamin fragments or truncated translation products.

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