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Fig. 1. Cellular localization of GFP/MEF2 fusion proteins. (A) Cellular localization of GFP (a), GFP/MEF2A (b) and GFP/MEF2C (c) in transfected C2.7 myoblasts; Hoechst staining of nuclei is shown in d-f: the arrows indicate the nuclei corresponding to green fluorescent cells. (B) Cellular localization of GFP (a), GFP/MEF2A{Delta}-471 (b), GFP/MEF2C{Delta}-429 (c), GFP/MEF2C{Delta}-411 (d), GFP/MEF2C{Delta}-399 (e) in transfected C2.7 myoblasts; Hoechst staining of nuclei is shown in panels f-j: the arrows indicate the nuclei corresponding to green fluorescent cells. Bar, 5 µm. (C) Linear representation of the GFP/MEF2 constructs used. The relative distribution of GFP fluorescence in the nuclei (N), in the cytoplasm (C), or in the nuclei and cytoplasm (N&C) of transfected C2.7 cells is shown for each construct. NLS refers to the C-terminal sequence encompassing aa 472-507 in MEF2A, or aa 430-466 in MEF2C, where the bipartite nuclear localization signal (NLS) is located.





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