Fig. 2. Cellular localization of GFP fused to selected C-terminal domains of MEF2A and MEF2C. (A) Cellular localization of GFP (a), GFP/MEF2A472-507 (b), GFP/MEF2C430-466 (c), GFP/MEF2C401-466 (d), GFP/MEF2C389-411 (e), GFP/MEF2C401-429 (f) in transfected C2.7 myoblasts. Bar, 5 µm. (B) Nuclear/cytoplasmic ratio of GFP fluorescence corresponding to the GFP/MEF2 constructs shown in A. (C) Linear representation of the GFP/MEF2 constructs used. NLS refers to the C-terminal sequence encompassing aa 472-507 in MEF2A or aa 430-466 in MEF2C, where the bipartite nuclear localization signal (NLS) is located. (D) Sequence alignment of the C-terminal region of human MEF2A and MEF2C. Amino acids corresponding to the bipartite nuclear localization signal are shown in bold. The sequence of MEF2C containing a putative nuclear retention signal is underlined.
