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Fig. 3. Cellular localization of HDAC4 in C2.7 cells co-transfected with MEF2 proteins. (A) Cellular localization of Myc-tagged HDAC4 and HA-tagged HDAC5 in proliferating C2.7 myoblasts (a and b, respectively) as well as in differentiated C2.7 myotubes (c and d, respectively); corresponding Hoechst stained nuclei are indicated by arrows in e-h. (B) Cellular localization of Myc-tagged HDAC4 (red) in C2.7 myoblasts co-transfected with pGFP/MEF2A (a), pGFP/MEF2C (b), pGFP/MEF2A{Delta}-471 (c), pGFP/MEF2C{Delta}-429 (d) and pGFP/MEF2C{Delta}-399 (e). Green fluorescence of the same cells co-expressing the indicated GFP/MEF2 fusion proteins is shown in f-j. Bar, 4 µm.





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