Fig. 1. Cloning and expression of rat nesprin-1. (A) Northern blot showing high expression of a 10 kb transcript of rat nesprin-1 (1RA1) in aortic tissue compared with cultured, passage 12, dedifferentiated VSMCs and fibroblasts. (B) Semi-quantitative RT-PCR analysis of rat nesprin-1 (1RA1), showing an increase in expression during developmental differentiation of the aorta. f17, foetal day 17; f19, foetal day 19; n1, neonatal day 1; w2-w8, weeks 2 to 8; ad, adult and c, negative control. The graph shows relative expression of 1RA1 at each stage normalized to a GAPDH control. (C) Schematic representation of isolation of rat nesprin-1 cDNA sequence.
