Fig. 2. Human nesprin-1 expression and gene analysis. (A) Northern blot of nesprin-1 expression in human tissues showing transcripts at 10.7 kb and 3.8 kb. The ß-actin loading control is shown. (B) Gene structure of human nesprin-1. The numbered boxes indicate exons, the horizontal lines indicate introns. Nesprin-1 comprises 19 exons, starting at exon 45 and excluding exon 47; nesprin-1ß comprises 61 exons (excluding exons 45, 47 and 55); nesprin-1₂ comprises18 exons and is initiated in exon 47, which contains an additional 31 N-terminal residues. (C) The deduced amino acid sequence of human nesprin-1 and -1ß. The first methionine of nesprin-1 and -1ß are shown in bold and underlined, the spectrin repeats (SR) are shown by numbered grey boxes. The open box indicates the TM region and the dashed open boxes indicate NLS. The amino acids encoded by exon 55 are shown in bold, whereas the open triangle indicates the insertion site of the 31 residues encoded by exon 47 (not shown). GenBank accession numbers for human nesprin-1 and 1ß are AY061756 and AY061755, respectively.

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