Fig. 1. (A) Immunoblot analysis of EGFP-rab5aQ79L induction. An EcR293 cell line stably transfected with pIND-Hygro/EGFP-rab5aQ79L (EcR293 pIND-Hygro/EGFP-rab5aQ79L) was treated for 24, 48, 72 or 96 hours with 5 µM ponasterone or with vehicle (veh: 0.125% ethanol) for 96 hours only. Total cell lysates were electrophoresed through SDS-PAGE, blotted and probed with a monoclonal anti-rab5 antibody. Endogenous rab5 migrates at approximately 25 kDa, and EGFP-rab5aQ79L at 50 kDa. (B) Deconvolution fluorescence microscopy of EcR293/EGFP-rab5aQ79L cells treated for 72 hours with 5 µM ponasterone (pon) or vehicle (veh).
