Fig. 6. Endocytic markers accumulate in EGFP-rab5aQ79L-containing vesicles. (A,B). EcR293pIND-Hygro /EGFP-rab5a wt cells (A) and EcR293pIND-Hygro/EGFP-rab5aQ79L cells (B) were induced with 5 µM ponasterone for 72 hours, then pulse labeled for 1 hour with dextran Texas Red, followed by a 6 hour chase prior to fixation. (C,D). EcR293 pIND-Hygro/EGFP-rab5a wt cells (C) and EcR293 pIND-Hygro/EGFP-rab5aQ79L cells (D) were induced with 5 µM ponasterone for 72 hours, then pulse labeled for 5 minutes with Di-LDL, followed by a 1 hour chase prior to fixation. The labeled cells were imaged by deconvolution fluorescence microscopy. Bar, 10 µm.
