Fig. 8. Dextran Texas Red preloaded into lysosomes localizes with EGFP-rab5aQ79L. Cells were incubated with dextran Texas Red (1 mg/ml) for 1 hour, washed and then chased for 6 hours in the absence of tracer. The cells were then induced with 5 µM ponasterone or vehicle for 72 hours prior to fixation, and imaged by deconvolution fluorescence microscopy. (A) EcR293 pIND-Hygro/EGFP-rab5a wt; (B) EcR293 pIND-Hygro/EGFP-rab5aQ79L; (C) EcR293 pIND-Hygro/EGFP-rab5aQ79L pulse labeled with dextran Texas Red and incubated 72 hours with vehicle alone. Bar, 10 µm.
