Fig. 9. Distribution of ß-hexosaminidase in Percoll gradients. EcR293 pIND-Hygro/EGFP-rab5aQ79L cells were treated with vehicle () or ponasterone () for 72 hours, then homogenized and applied to self-forming Percoll gradients (see Materials and Methods). After centrifugation, samples were taken from the bottom (fraction 1), and aliquots assayed for ß-hexosaminidase activity and protein concentration. Results are representative of three independent experiments.

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