Fig. 1. (A) P95-APP1-derived constructs used in this study. Zn, zinc finger; GAP, ARF-GAP domain; ANK, ankyrin repeats; SHD, Spa2 homology 1 domain; COIL, coiled-coil region; PBS, paxillin binding subdomain; K39 indicates the amino acid substitution in the ARF-GAP domain of the p95-K39 construct. (B) Coimmunoprecipitation of p95-C2 with paxillin and PIX. Aliquots of a lysate from CEFs transfected with pFlag-p95-C2 (lanes 1-6) were immunoprecipitated with the mAb anti-Flag and blotted with anti-Flag to detect p95-C2 (lanes 1-3) or with anti-paxillin (lanes 4-6). Lysate from CEFs cotransfected with pFlag-p95-C2 and pXJ40-HA-ßPIX (lanes 7-9) were immunoprecipitated with the anti-Flag mAb. Immunoprecipitates were blotted with the anti-Flag mAb to detect p95-C2 (bottom panel) and with the anti-HA mAb to detect the coprecipitating ß-PIX polypeptide (top panel). Pre, beads from pre-clearing; IP, immunoprecipitates; nb, material not bound to beads. Molecular weight markers are indicated to the left of panels.
