Fig. 2. Subcellular localization of p95-C2, ß-PIX, PAK1 and p95-N4. (A-J) Localization of p95-C2, ß-PIX and PAK1 at large vesicles. Cells were cotransfected with pFlag-p95-C2 (A,C) and pCMV6m/Pak1 (B,D), or with pFlag-p95-C2 (F) and pXJ40-HA-ßPIX (G). (E) Cell transfected with CMV6m/Pak1 only. (H) Cell transfected with pXJ40-HA-ßPIX only. In (I,J) cells were cotransfected with pXJ40-HA-ßPIX (I) and pCMV6m/Pak1 (J). (K-O) Localization of p95-N4 at small vesicles. Cells were cotransfected with pFlag-p95-N4 (K) and pCMV6m/Pak1 (L), and with pFlag-p95-N4 (M) and pXJ40-HA-ßPIX (N). In (O), merged signals for p95-N4 (shown in red) and ß-PIX (shown in green) are shown. Cells were fixed one day after transfection and processed for indirect immunofluorescence as described in Materials and Methods. Bar, 10 µm.
