Fig. 7. Effects of p95-C, p95-N5, and p95-C5 on cell morphology. Fibroblasts transfected with pFlag-p95-C, pFlag-p95-N5, or pFlag-p95-C5 were used for immunofluorescence. (A,B) The effects of each construct on the protrusive activity of the transfected cells was quantified as described in Materials and Methods, and compared to the protrusive activity of non-transfected cells (Control). (A) Percentage of cells with protrusions. (B) Number of protrusions per cell. For each value, three independent experiments were examined; bars represent the s.e.m. (C) Cells were transfected with pFlag-p95-N5 (a,c,f,h,j), with pFlag-p95-C (b), or with pFlag-p95-C5 (l) and treated for immunofluorescence one day after transfection. The distribution of p95-N5 was compared with the distribution of endogenous Rab11 (d,i,k), and of endogenous EEA1 (g). In (e) the signal for p95-N5 is shown in green, Rab11 is shown in red. Bars, 10 µm (a-i,l,m); 5 µm (j,k). Arrows point to corresponding areas of the cytoplasm in j and k.
