Fig. 9. Relocalization of p95-C2, p95-C3, paxillin, PIX, and PAK to the cell periphery by activated Rac. Fibroblasts were transfected or cotransfected with the following plasmids: pFlag-p95-C2 (A); pFlag-p95-C2 and pFlag-V12-Rac1B (B,C,E,L,M,P,Q); pFlag-p95-C2 and pFlag-N17-Rac1B (D,F); pFlag-p95-C3 and pFlag-V12-Rac1B (G-I,N,O); pFlag-p95-N4 and pFlag-V12-Rac1B (J,K); pFlag-p95-C3, pCMV6m/Pak1, and pFlag-V12-Rac1B (R,S); pFlag-p95-C3, pXJ40-HA-ßPIX, and pFlag-V12-Rac1B (T,U). Cells were fixed and processed for indirect immunofluorescence one day after transfection. The same field is shown in (C,E), (D,F), (G,H), (J,K), (L,M), (N,O), (P,Q), (R,S), and (T,U). Bars, 10 µm. V12-Rac induces the localization of both p95-C2 (B,C,E) and p95-C3 (G,H) at the cell periphery, together with paxillin (L-O), Pak (R, S), and Pix (T,U). V12-Rac does not influence the localization of p95-N4 (J,K).
