Fig. 10. Overexpression of Arl1(Q71L) transforms the Golgi into an extensive vesicular-tubular network. Pooled Arl1(Q71L)-transfected CHO cells were infected with VSVts045 at 32°C for one hour and followed by two hours in the absence and one hour in the presence of 10 µg/ml cycloheximide. Cryosections derived from these cells were processed for immunogold EM to detect Arl1(Q71L) and VSV-G. In cells expressing moderate levels of Arl1(Q71L) (upper panel), Golgi cisternae became more dilated and expanded. 9 nm and 14 nm gold particles represent Arl1(Q71L) and VSV-G (arrowheads), respectively. In cells expressing high levels of Arl1(Q71L) (lower panel), the Golgi apparatus was transformed into an extensive tubular-vesicular network. Arl1(Q71L) (9 nm gold particles, small arrowheads) decorated the entire network, with VSV-G (14 nm gold particles, large arrowheads) being accumulated in the structure. Bars: 100 nm.
