Fig. 4. The GDP-bound form of Arl1 disassembles the Golgi apparatus. CHO (A-C) or A431 cells (D-L) were transiently transfected with a construct expressing Arl1(T31N) (A-C and G-L) or Arl1(T31N)-EGFP (D-F), in which Thr at position 31 was mutated to Asn. Cells were then processed for double labeling using a limiting amount of Arl1 antibody (A, G and J) and antibodies against GS28 (B), GT(ß-1,4-galactosyltransferase) (E), 
-adaptin (H) or p115 (K). The Golgi labeling of GS28 and GT were disrupted in cells overexpressing Arl1(T31N) (A-F). -adaptin (AP-1) was dissociated from perinuclear Golgi structure in Arl1(T31N)-expressing cells (G-I). Overexpression of Arl1(T31N) did not affect the Golgi-like distribution of p115, a Golgi-matrix protein (J-L). Bars: 10 µm.
