Fig. 5. Expression of the GTP-bound form of Arl1 caused an expansion of the Golgi. Pooled Arl1(Q71L) transfected CHO cells were double labeled with a limiting amount of Arl1 antibody to label exogenous Arl1(Q71L) and antibody against GS28 (A-C). Arl1(Q71L) was associated with Golgi (A) and caused an expansion of Golgi apparatus marked by GS28 (B). CHO cells were transiently transfected with constructs expressing Arl1(Q71L)-EGFP (D-F) or Arl1(G2A, Q71L)-EGFP (G-I). Cells were then processed to reveal expressed protein as well as endogenous GS28. In contrast to Arl1(Q71L)-EGFP, Arl1(G2A, Q71L)-EGFP was not detected in the Golgi apparatus and had no effect on the Golgi marked by GS28 (H). Bars: 10 µm.
